We have just published our paper on fully quantitative imaging of ryanodine receptors in Cell Reports. A good start to 2018! Using DNA based labeling we were able to push the optical resolution to new limits.
Here are our highlights:
- With enhanced resolution, individual RyRs were observed within dense clusters
- RyRs appear to assemble randomly and unconstrained
- The ratio of RyR co-clustering with JPH2 varies considerably between nanodomains
- DNA-based super-resolution enables fully quantitative imaging at 10 nm resolution
Check out the full text, it is open access under a CC BY license!
RyRs at molecular resolution using the powerful virtual magnifying glass of optical super-resolution.